Skip to content
naulon
Get started

No retraction, correction or concern found when last checked on Nov 1, 2026.

Allogeneic Periodontal Ligament Stem Cell Therapy for Periodontitis in Swine

Gang Ding, Yi Liu, Wei Wang and 6 more

Stem Cells · 2010

doi:10.1002/stem.512

Abstract

Periodontitis is one of the most widespread infectious diseases in humans. It is the main cause of tooth loss and associated with a number of systemic diseases. Until now, there is no appropriate method for functional periodontal tissue regeneration. Here, we establish a novel approach of using allogeneic periodontal ligament stem cells (PDLSCs) sheet to curing periodontitis in a miniature pig periodontitis model. Significant periodontal tissue regeneration was achieved in both the autologous and the allogeneic PDLSCs transplantation group at 12 weeks post-PDLSCs transplantation. Based on clinical assessments, computed tomography (CT) scanning, and histological examination, there was no marked difference between the autologous and allogeneic PDLSCs transplantation groups. In addition, lack of immunological rejections in the animals that received the allogeneic PDLSCs transplantation was observed. Interestingly, we found that human PDLSCs fail to express human leukocyte antigen (HLA)-II DR and costimulatory molecules. PDLSCs were not able to elicit T-cell proliferation and inhibit T-cell proliferation when stimulated with mismatched major histocompatibility complex molecules. Furthermore, we found that prostaglandin E2 (PGE2) plays a crucial role in PDLSCs-mediated immunomodulation and periodontal tissue regeneration in vitro and in vivo. Our study demonstrated that PDLSCs possess low immunogenicity and marked immunosuppression via PGE2-induced T-cell anergy. We developed a standard technological procedure of using allogeneic PDLSCs to cure periodontitis in swine.

Shown for reading. Its publisher allows quoting it, not republishing it.

References 34

  1. 1.

    Kinane DF, Marshall GJ. Periodontal manifestations of systemic disease. Aust Dent J. 2001;46:2-12.

    Not in our library10.1111/j.1834-7819.2001.tb00267.x

  2. 2.
    Periodontal Tissue Regeneration Using Fibroblast Growth Factor -2: Randomized Controlled Phase II Clinical Trial · 2008
    No notice
  3. 3.

    Nygaard-Østby P, Bakke V, Nesdal O, et al. Periodontal healing following reconstructive surgery: Effect of guided tissue regeneration using a bioresorbable barrier device when combined with autogenous bone grafting. A randomized-controlled Trial 10-year follow-up. J Clin Periodontol. 2010;37:366-373.

    Not in our library10.1111/j.1600-051x.2010.01532.x

  4. 4.

    Lee JS, Wikesjö UM, Jung UW, et al. Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a beta-tricalcium phosphate carrier into one-wall intrabony defects in dogs. J Clin Periodontol. 2010;37:382-389.

    Not in our library10.1111/j.1600-051x.2010.01544.x

  5. 5.

    Villar CC, Cochran DL. Regeneration of periodontal tissues: Guided tissue regeneration. Dent Clin North Am. 2010;54:73-92.

    Not in our library10.1016/j.cden.2009.08.011

  6. 6.

    Seo BM, Miure M, Gronthos S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364:149-155.

    Not in our library10.1016/s0140-6736(04)16627-0

  7. 7.

    Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97:13625-13630.

    Not in our library10.1073/pnas.240309797

  8. 8.

    Miura M, Gronthos S, Zhao M, et al. SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 2003;100:5807-5812.

    Not in our library10.1073/pnas.0937635100

  9. 9.

    Liu Y, Zheng Y, Ding G, et al. Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells. 2008;26:1065-1073.

    Not in our library10.1634/stemcells.2007-0734

  10. 10.
    Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine · 2006
    No notice
  11. 11.

    Shi S, Gronthos S, Chen S, et al. Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression. Nat Biotechnol. 2002;20:587-591.

    Not in our library10.1038/nbt0602-587

  12. 12.

    Hasegawa M, Yamato M, Kikuchi A, et al. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. Tissue Eng. 2005;11:469-478.

    Not in our library10.1089/ten.2005.11.469

  13. 13.

    Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 2003;18:696-704.

    Not in our library10.1359/jbmr.2003.18.4.696

  14. 14.

    Sacchetti B, Funari A, Michienzi S, et al. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell. 2007;131:324-336.

    Not in our library10.1016/j.cell.2007.08.025

  15. 15.

    Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society For Cellular Therapy Position Statemen. Cytotherapy. 2006;8:315-317.

    Not in our library10.1080/14653240600855905

  16. 16.

    Ding G, Wang W, Liu Y, et al. Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla. J Cell Physiol. 2010;223:415-422.

    Not in our library10.1002/jcp.22050

  17. 17.

    Di Nicola M, Carlo-Stella C, Magni M, et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood. 2002;99:3838-3843.

    Not in our library10.1182/blood.v99.10.3838

  18. 18.

    Krampera M, Glennie S, Dyson J, et al. Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide. Blood. 2003;101:3722-3729.

    Not in our library10.1182/blood-2002-07-2104

  19. 19.

    Beyth S, Borovsky Z, Mevorach D, et al. Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T- cell unresponsiveness. Blood. 2005;105:2214-2219.

    Not in our library10.1182/blood-2004-07-2921

  20. 20.

    Aggarwal S, Pittinger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2005;105:1815-1822.

    Not in our library10.1182/blood-2004-04-1559

  21. 21.

    Wada N, Menicanin D, Shi S, et al. Immunomodulatory properties of human periodontal ligament stem cells. J Cell Physiol. 2009;219:667-676.

    Not in our library10.1002/jcp.21710

  22. 22.

    Le Blanc K, Rasmusson I, Sundberg B, et al. Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet. 2004;363:1439-1441.

    Not in our library10.1016/s0140-6736(04)16104-7

  23. 23.

    Uccelli A, Pistoia V, Moretta L. Mesenchymal stem cells: A new strategy for immunosuppression? Trends Immunol. 2007;28:219-226.

    Not in our library10.1016/j.it.2007.03.001

  24. 24.

    Sun LY, Akiyama K, Zhang HY, et al. Mesenchymal stem cell transplantation reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans. Stem Cells. 2009;27:1421-1432.

    Not in our library10.1002/stem.68

  25. 25.

    Ding G, Liu Y, Wang W, et al. Suppression of T cell proliferation by root apical papilla stem cells in vitro. Cells Tissues Organs. 2010;191:357-364.

    Not in our library10.1159/000276589

  26. 26.

    Haniffa MA, Wang XN, Holtick U, et al. Adult human fibroblasts are potent immunoregulatory cells and functionally equivalent to mesenchymal stem cells. J Immunol. 2007;179:1595-1604.

    Not in our library10.4049/jimmunol.179.3.1595

  27. 27.

    Jones S, Horwood N, Cope A, et al. The antiproliferative effect of mesenchymal stem cells is a fundamental property shared by all stromal cells. J Immunol. 2007;179:2824-2831.

    Not in our library10.4049/jimmunol.179.5.2824

  28. 28.

    Gaffen SL, Hajishengallis G. A new inflammatory cytokine on the block: Re-thinking periodontal diseases and the Th1/Th2 paradigm in the context of Th17 cells and IL-17. J Dent Res. 2008;87:817-828.

    Not in our library10.1177/154405910808700908

  29. 29.

    Wang S, Liu Y, Fang D, et al. The miniature pig: A useful large animal model for dental and orofacial research. Oral Dis. 2007;13:530-537.

    Not in our library10.1111/j.1601-0825.2006.01337.x

  30. 30.

    Yanez R, Lamana ML, Garcia-Castro J, et al. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease. Stem Cells. 2006;24:2582-2591.

    Not in our library10.1634/stemcells.2006-0228

  31. 31.

    Kubo M, Sonoda Y, Muramatsu R, et al. Immunogenicity of human amniotic membrane in experimental xenotransplantation. Invest Ophthalmol Vis Sci. 2001;42:1539-1546.

    Not in our library

  32. 32.

    Kim SH, Kim KH, Seo BM, et al. Alveolar bone regeneration by transplantation of periodontal ligament stem cells and bone marrow stem cells in a canine peri-implant defect model: A pilot study. J Periodontol. 2006;77:1003-1007.

    Not in our library10.1902/jop.2009.090249

  33. 33.

    Kawaguchi H, Hirachi A, Hasegawa N, et al. Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells. J Periodontol. 2004;75:1281-1287.

    Not in our library10.1902/jop.2004.75.9.1281

  34. 34.

    Li H, Yan F, et al. Application of autologous cryopreserved bone marrow mesenchymal stem cells for periodontal regeneration in dog. Cells Tissues Organs. 2009;190:94-101.

    Not in our library10.1159/000166547

Cited by papers we hold 9

Reads

Read by people on this page

None yet.

Checked or read by AI through naulon

None yet.

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.

Are you an author?

Is this your paper?

Claim it to add your note and be paid when agents cite it. Free.