Magnetic immobilization of a quorum sensing signal hydrolase, AiiA
Lin Wang, Haixing Xu, Zewen Liu and 5 more
MicrobiologyOpen · 2019
doi:10.1002/mbo3.797 (opens in a new tab)
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Abstract
Abstract Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3O4‐SiO2 iron beads and was confirmed via SDS‐PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6‐HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.
Abstract licence: CC BY-NC
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