MicroRNA‐379 inhibits laryngeal carcinoma cell proliferation and invasion through directly targeting TCF‐4

Abstract The expression pattern, functions, and detailed regulatory mechanisms of miR‐379 in laryngeal carcinoma remain unknown. In the present study, we aimed to uncover novel potential miR‐379 targets and shed light on its roles in laryngeal carcinoma. The expression level of miR‐379 was measured based on the data obtained from the TCGA database and the cell lines. After miR‐379 mimic transfection, cell proliferation, invasion and migration assay, and wound‐healing assay in HEp‐2 cell line were implemented to evaluate the effects of miR‐379 on laryngeal carcinoma in vitro. The target genes for miR‐379 in silico were predicted and validated using luciferase reporter assay. The miR‐379 expression level was reduced in laryngeal carcinoma tissues and cell lines. Moreover, miR‐379 overexpression inhibited the cell proliferation, migration, and invasion of laryngeal carcinoma cells. TCF‐4 was detected as a direct target of miR‐379 in laryngeal carcinoma. Furthermore, restored TCF‐4 expression rescued the inhibitory roles of miR‐379 overexpression on cell proliferation, migration, and invasion.Our study for the first time demonstrated that miR‐379/TCF‐4 might involve in the progression of laryngeal carcinoma, and miR‐379 appears to serve as a novel tumor suppressor in laryngeal carcinoma.

Abstract licence: CC BY-NC-ND

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