Understanding of the impact of chemicals on amphibians: a meta‐analytic review

Abstract

Many studies have assessed the impact of different pollutants on amphibians across a variety of experimental venues (laboratory, mesocosm, and enclosure conditions). Past reviews, using vote‐counting methods, have described pollution as one of the major threats faced by amphibians. However, vote‐counting methods lack strong statistical power, do not permit one to determine the magnitudes of effects, and do not compare responses among predefined groups. To address these challenges, we conducted a meta‐analysis of experimental studies that measured the effects of different chemical pollutants (nitrogenous and phosphorous compounds, pesticides, road deicers, heavy metals, and other wastewater contaminants) at environmentally relevant concentrations on amphibian survival, mass, time to hatching, time to metamorphosis, and frequency of abnormalities. The overall effect size of pollutant exposure was a medium decrease in amphibian survival and mass and a large increase in abnormality frequency. This translates to a 14.3% decrease in survival, a 7.5% decrease in mass, and a 535% increase in abnormality frequency across all studies. In contrast, we found no overall effect of pollutants on time to hatching and time to metamorphosis. We also found that effect sizes differed among experimental venues and among types of pollutants, but we only detected weak differences among amphibian families. These results suggest that variation in sensitivity to contaminants is generally independent of phylogeny. Some publication bias (i.e., selective reporting) was detected, but only for mass and the interaction effect size among stressors. We conclude that the overall impact of pollution on amphibians is moderately to largely negative. This implies that pollutants at environmentally relevant concentrations pose an important threat to amphibians and may play a role in their present global decline.

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References 133

1. 1.

   Abouheif E. A method to test the assumption of phylogenetic independence in comparative data. Evol. Ecol. Res. 1999;1:895-909.

   Not in our library

2. 2.

   Altwegg R, Reyer HU. Patterns of natural selection on size at metamorphosis in water frogs. Evolution. 2003;57:872-882.

   Not in our library10.1111/j.0014-3820.2003.tb00298.x

3. 3.

   Bancroft BA, Baker NJ, Blaustein AR. Effects of UV-B radiation on marine and freshwater organims: a synthesis through meta-analysis. Ecol. Lett. 2007;10:332-345.

   Not in our library10.1111/j.1461-0248.2007.01022.x

4. 4.

   Bancroft BA, Baker NJ, Blaustein AR. A meta-analysis of the effects of ultraviolet B radiation and its synergistic interactions with pH, contaminants, and disease on amphibian survival. Conserv. Biol. 2008;22:987-996.

   Not in our library10.1111/j.1523-1739.2008.00966.x

5. 5.

   Beebee TJC, Griffiths RA. The amphibian decline crisis: a watershed for conservation biology? Biol. Conserv. 2005;125:271-285.

   Not in our library

6. 6.

   Begg CB, Mazumdar M. Operating characteristics of a rank correlation test for publication bias. Biometrics. 1994;50:1088-1101.

   Not in our library

7. 7.

   Begg CB, Cooper H, Hedges V. Publication bias. The handbook of research synthesis. 1994;399-409.

   Not in our library

8. 8.

   Berrill M, Bertram S. Effects of pesticides on amphibian embryos and larvae. Herpetol. Conserv. 1997;1:233-245.

   Not in our library

9. 9.

   Berrill M, Coulson D, Mc Gillivray L, Pauli B. Toxicity of endosulfan to aquatic stages of anuran amphibians. Environ. Toxicol. Chem. 1998;17:1738-1744.

   Not in our library

10. 10.

    Berrill M, Bertram S, Mc Gillivray L, Kolohon M, Pauli B. Effects of low concentrations of forest use pesticides on frog embryos and tadpoles. Environ. Toxicol. Chem. 1994;13:657-664.

    Not in our library

11. 11.

    Berven KE, Gill DE. Interpreting geographic variation in life-history traits. Am. Zool. 1983;23:85-93.

    Not in our library

12. 12.

    Boone MD, Bridges-Britton C. Examining multiple sublethal contaminants on the gray treefrog (Hyla versicolor): effects of an insecticide, herbicide and fertilizer. Environ. Toxicol. Chem. 2006;25:3261-3265.

    Not in our library10.1897/06-235r.1

13. 13.

    Boone MD, Bridges CM, Semlitsch RD. Effects of pesticides on amphibian populations. Amphibian conservation. 2003;152-167.

    Not in our library

14. 14.

    Boone MD, James D. Aquatic and terrestrial mesocosms in amphibian ecotoxicology. Appl. Herpetol. 2005;2:231-257.

    Not in our library

15. 15.

    Boone MD, Bridges CM, Fairchild JF, Little EE. Multiple sublethal chemicals negatively affect tadpoles of the green frog, Rana clamitans. Environ. Toxicol. Chem. 2005;24:1267-1272.

    Not in our library10.1897/04-319r.1

16. 16.

    Boone MD, Semlitsch RD, Little EE, Doyle MC. Multiple stressors in amphibian communities: effects of chemical contamination, bullfrogs, and fish. Ecol. Appl. 2007;17:291-301.

    Not in our library10.1890/1051-0761(2007)017[0291:msiace]2.0.co;2

17. 17.

    Bridges CM, Semlitsch RD. Variation in pesticide tolerance of tadpoles among and within species of Ranidae and patterns of amphibian decline. Conserv. Biol. 2000;14:1490-1499.

    Not in our library

18. 18.

    Bridges CM. Tadpole swimming performance and activity affected by acute exposure to sublethal levels of carbaryl. Environ. Toxicol. Chem. 1997;19:1935-1939.

    Not in our library

19. 19.

    Bridges CM. Effects of a pesticide on tadpole activity and predator avoidance behavior. J. Herpetol. 1999;33:303-306.

    Not in our library

20. 20.

    Bridges CM. Long-term effects of pesticide exposure at various life stages of the Southerm leopard frog (Rana sphenocephala. Arch. Environ. Contam. Toxicol. 2000;39:91-96.

    Not in our library10.1007/s002440010084

21. 21.

    Bucciarelli T, Sacchetta P, Pennelli A, Cornelio L, Romagnoli R, Merino S, Petruzelli R, Di Ilio C. Characterization of toad glutathione transferase. Biochim. Biophys. Acta. 1999;1431:189-198.

    Not in our library10.1016/s0167-4838(99)00036-9

22. 22.

    Camargo JA, Alonso A, Salamanca A. Nitrate toxicity to aquatic animals: a review with new data for freshwater invertebrates. Chemosphere. 2005;58:1255-1267.

    Not in our library10.1016/j.chemosphere.2004.10.044

23. 23.

    Camargo JA, Alonso A. Ecological and toxicological effects of inorganic nitrogen pollution in aquatic ecosystems: a global assessment. Environ. Int. 2006;32:831-849.

    Not in our library10.1016/j.envint.2006.05.002

24. 24.

    Carpenter S, Caraco NF, Correll DL, Howarth RW, Sharpley AN, Smith VH. Nonpoint pollution of surface waters with phosphorous and nitrogen. Ecol. Appl. 1998;8:559-568.

    Not in our library

25. 25.

    Chalcraft DR, Binckley CA, Resetarits WJ. Experimental venue and estimation of interaction strength: comment. Ecology. 2005;86:1061-1067.

    Not in our library

26. 26.

    Chen TH, Gross JA, Karasov WH. Sublethal effects of lead on Northern leopard frog (Rana pipiens) tadpoles. Environ. Toxicol. Chem. 2006;25:1383-1389.

    Not in our library10.1897/05-356r.1

27. 27.

    Christensen MR, Graham MD, Vinebrooke RD, Findlay DL, Paterson MJ, Turner MA. Multiple anthropogenic stressors cause ecological surprises in boreal lakes. Glob. Change Biol. 2006;12:1-7.

    Not in our library

28. 28.

    Cohen J. Statistical power analysis for the behavioral sciences. 1969.

    Not in our library

29. 29.

    Accumulation of Nitrate. 1972.

    Not in our library

30. 30.

    Cowman DF, Mazanti LE, Sparling DW, Linder G, Bishop CA. Ecotoxicology of “new generation” pesticides to amphibians. Ecotoxicology of amphibians and reptiles. 2000;233-268.

    Not in our library

31. 31.

    Daszak P, Cunningham AA, Hyatt AD. Anthropogenic environmental change and the emergence of infectious diseases in wildlife. Acta Tropica. 2001;78:103-116.

    Not in our library10.1016/s0001-706x(00)00179-0

32. 32.

    Davidson C, Shaffer HB, Jennings MR. Declines of the California red-legged frog: climate, UV-B, habitat and pesticides hypotheses. Ecol. Appl. 2001;11:464-479.

    Not in our library

33. 33.

    Dayton GH, Fitzgerald LA. Competition, predation, and the distributions of four desert anurans. Oecologia. 2001;129:430-435.

    Not in our library10.1007/s004420100727

34. 34.

    Duellman W, Trueb L. Biology of amphibians. 1994.

    Not in our library

35. 35.

    Dunson WA, Connell J. Specific inhibition of hatching in amphibians exposed to low pH. J. Herpetol. 1982;16:314-316.

    Not in our library

36. 36.

    Edginton AN, Rouleau C, Stephenson GR, Boermans HJ. 2,4-D butoxyethyl ester kinetics in embryos of Xenopus laevis: the role of the embryonic jelly coat in reducing chemical absorption. Arch. Environ. Contam. Toxicol. 2007;52:113-120.

    Not in our library10.1007/s00244-005-0215-4

37. 37.

    Egea-Serrano A, Tejedo M, Torralva M. Estimating mean lethal concentrations of three nitrogenous compounds for larvae of the Iberian waterfrog, Pelophylax perezi (Seoane, 1885). Revista Española de Herpetología. 2009a;23:107-116.

    Not in our library

38. 38.

    Egea-Serrano A, Tejedo M, Torralva M. Populational divergence in the impact of three nitrogenous compounds and their combination on larvae of the frog Pelophylax perezi (Seoane, 1885). Chemosphere. 2009b;76:869-877.

    Not in our library10.1016/j.chemosphere.2009.05.017

39. 39.

    Egea-Serrano A. 2010;366.

    Not in our library

40. 40.

    Faivovich J, Haddad CFB, Garcia PCA, Frost DR, Campbell JA, Wheeler WC. Systematic review of the frog family Hylidae, with special reference to Hylinae: phylogenetic analysis and taxonomic revision. Bull. Am. Mus. Nat. Hist. 2005;294:1-240.

    Not in our library

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