Chemical Synthesis of Ubiquitin, Ubiquitin‐Based Probes, and Diubiquitin

Abstract

Changing the subject: An efficient linear solid-phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C-terminal modification and the construction of all diubiquitin conjugates in high yields and purities in a straightforward manner.

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Cited by papers we hold 3

• Diubiquitin-Based NMR Analysis: Interactions Between Lys6-Linked diUb and UBA Domain of UBXN1 · 2020
  Not checked
• BAP1/ASXL1 recruitment and activation for H2A deubiquitination · 2016
  No notice
• K29-Selective Ubiquitin Binding Domain Reveals Structural Basis of Specificity and Heterotypic Nature of K29 Polyubiquitin · 2015
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